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The frequency of rs776746 polymorphism in the CYP3A5 gene in women with non-developing pregnancy

https://doi.org/ 10.24411/2588-0527-2018-10005

Abstract

Purpose. The purpose of this study was to examine frequency of genetic polymorphism of CYP3A5*3 (rs776746 A>T in women with non-developing pregnancy. Methodology. The study included all women admitted to the Department of obstetrics and gynecology of the Peter Great clinic with a verified diagnosis of non-developing pregnancy. In parallel, the control group included women with normal pregnancy, comparable in age and the presence or absence of comorbidities. After obtaining consent from women, peripheral venous blood was collected for genetic research. Gen CYP3A5*3/*1 (6986A>G) polymorphism was determined by real-time polymerase chain reaction method using the developed sets («Synthol», Russia). Comparison of the detected allele frequencies was performed using Chi-square method. The Graphpad Prism 5.0 statistical program was used. Results. 53 persons were included in the study group of women with non-developing pregnancy. The control group, comparable in age and comorbidity, included 92 people. The frequency of minor allele Т in the study group was 8 %, in the control group - 4 % (p = 0.1; 2). The distribution of genotypes in the main group was as follows: ТТ - 0.02; TC - 0.13; CC - 0.85. The distribution of genotypes in the control group: ТТ - 0.01; TC - 0.05; CC to 0.94. The distributions corresponded to the Hardy-Weinberg equation. Conclusion. The results of the study indicate a tendency to a higher frequency of minor allele Т in the group of women with non-developing pregnancy compared to women whose pregnancy was normal at the time of the study. The obtained data may indicate a possible role of CYP3A5 activity in the pathogenesis of non-developing pregnancy, or in the effectiveness of drug therapy, since this enzyme performs the function of endogenous metabolism of a number of sex hormones (progesterone, testosterone).

About the Authors

K. A. Zagorodnikova
Central research laboratory, North-Western State Medical University named after I.I. Mechnikov
Russian Federation


Yu. G. Rustanovich
Saint-Petersburg GBUZ GP No. 6, women’s consultation No. 31
Russian Federation


D. F. Kostyuchek
North-Western State Medical University named after I.I. Mechnikov
Russian Federation


A. A. Murzina
Central research laboratory, North-Western State Medical University named after I.I. Mechnikov
Russian Federation


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For citations:


Zagorodnikova K.A., Rustanovich Yu.G., Kostyuchek D.F., Murzina A.A. The frequency of rs776746 polymorphism in the CYP3A5 gene in women with non-developing pregnancy. Pharmacogenetics and Pharmacogenomics. 2018;(1):27-30. (In Russ.) https://doi.org/ 10.24411/2588-0527-2018-10005

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ISSN 2588-0527 (Print)
ISSN 2686-8849 (Online)